BREAKFAST WITH CORPSES
me
leaning
over the sink
spooning microwaved remnants
soup from the bakery three blocks away
watching the trees move green in mid October,
if tinted in spots by tan silk shrouded bodies tied
into the window screen the spider’s lardered remnants
of summer’s tender winged swarm if they’re termites some day
a sigh I never realized I even uttered will tear out the last
celulose column supporting my walls and we will
spill like sand into the basement
my microwave, window
soup carton,
spoon
me
I can’t get this quite right.
memory steps lightly
still feather gray on the leaf
is not the mockingbird
////
memory stumbles
one dew-touched gray feather
no mockingbird song
mediated destruction can be
intimately
coupled
1, 2 et al.
3 challenged Gal4
not dynamically, but rather
‘in’
competition.
Here evidence the unsuitable,
bound, Gal4 susceptible in vivo.
key evidence that
et al.3 used Gal4
within the context of
promoter complexes.
http://www.nature.com/nature/journal/v461/n7265/full/nature08406.html
Growing evidence supports the notion that proteasome-mediated destruction of transcriptional activators can be intimately coupled to their function1, 2. Recently, Nalley et al.3 challenged this view by reporting that the prototypical yeast activator Gal4 does not dynamically associate with chromatin, but rather ‘locks in’ to stable promoter complexes that are resistant to competition. Here we present evidence that the assay used to reach this conclusion is unsuitable, and that promoter-bound, active Gal4 is indeed susceptible to competition in vivo. Our data challenge the key evidence that Nalley et al.3 used to reach their conclusion, and indicate that Gal4 functions in vivo within the context of dynamic promoter complexes.
